Semrock & Chroma equivalents

Closest published brand part for each of our filters, with the key specification differences stated plainly. Our 5+ unit prices are about half (typically 35–45%) of the published list price of the closest brand part; single-unit prices are higher. Brand parts are premium products — choose what fits your application.

Our filters compared to the closest Semrock and Chroma parts
Our SKUClosest Semrock partClosest Chroma partOur key specsKey differences (honest notes)
DX-SET-DAPI
DAPI fluorescence filter set
DAPI-5060C-000 49000 ET-DAPI
  • Exciter: 365/40 nm (CWL 365 nm, bandwidth 40 nm); T 90%; OD6; blocking 200–800 nm
  • Emitter: 470/30 nm (CWL 470 nm, bandwidth 30 nm); T 92%; OD6; blocking 200–900 nm
  • Dichroic: Long-pass 410 nm; reflects 350–390 nm (R > 90%); transmits 420–750 nm (T > 90%)
  • Typical measured (from curve): 50% points: exciter ≈ 344–382 nm; emitter ≈ 454–483 nm; dichroic edge ≈ 412 nm
Cube format must match your microscope (Nikon / Olympus / Zeiss / Leica). A version with a long-pass emitter is also available on request.
DX-SET-GFP
GFP / FITC / FAM fluorescence filter set
GFP-3035D-000 49002 ET-EGFP (FITC/Cy2)
  • Exciter: 470/30 nm
  • Emitter: 525/20 nm
  • Dichroic: Long-pass 490 nm
  • Blocking: OD6; OD7 excitation/emission cross-blocking
  • Typical measured (from curve): 50% points: exciter ≈ 456–489 nm; emitter ≈ 515–537 nm; dichroic edge ≈ 502 nm; passband T ≈ 93–99%
Cube format must match your microscope.
DX-SET-TRITC
TRITC / Cy3 fluorescence filter set
Cy3-4040C-000 / TRITC-B 49004 ET-Cy3/TRITC
  • Exciter: 543/20 nm (CWL 543 nm, bandwidth 20 nm); T 95%; OD6; blocking 200–900 nm
  • Emitter: 585/20 nm (CWL 585 nm, bandwidth 20 nm); T 95%; OD6; blocking 200–900 nm
  • Cross-blocking: OD7 (exciter vs emitter)
  • Dichroic: Long-pass 560 nm; reflects 500–550 nm (R 95%); transmits 570–800 nm (T 95%)
  • Typical measured (from curve): 50% points: exciter ≈ 531–553 nm; emitter ≈ 572–594 nm; dichroic edge ≈ 560 nm
Cube format must match your microscope.
DX-SET-A488
Alexa Fluor 488 fluorescence filter set
FITC-5050A-000 49002 ET-EGFP (FITC/Cy2)
  • Exciter: 480/30 nm
  • Emitter: 527/20 nm
  • Dichroic: Long-pass 495 nm
  • Blocking: OD6
  • Typical measured (from curve): 50% points: exciter ≈ 466–497 nm; emitter ≈ 515–544 nm; dichroic edge ≈ 504 nm; passband T ≈ 92–97%
Cube format must match your microscope.
DX-SET-ICG
ICG (indocyanine green) fluorescence filter set
ICG-B-000 —
  • Exciter: 770/50 nm (CWL 770 nm, bandwidth 50 nm); T 90%; OD6; blocking 200–900 nm
  • Emitter: 845/50 nm (CWL 845 nm, bandwidth 50 nm); T 90%; OD6; blocking 200–900 nm
  • Dichroic: Long-pass 800 nm; reflects 630–790 nm (R 90%); transmits 820–1000 nm (T 90%)
  • Typical measured (from curve): 50% points: exciter ≈ 741–788 nm; emitter ≈ 819–872 nm; dichroic edge ≈ 811 nm
No directly comparable Chroma set listed in our reference data.
DX-SET-TXR-E
Texas Red / ROX economy fluorescence filter set
TxRed-4040C-000 49008 ET-mCherry/Texas Red
  • Blocking: OD5 (economy grade)
  • Typical measured (from curve): Exciter ≈ 560–581 nm at 50% (peak T ≈ 90%); emitter ≈ 621–647 nm at 50% (peak T ≈ 95%); dichroic long-pass edge ≈ 595 nm
Economy set with OD5 blocking. The dichroic shows some transmission below 545 nm (see curve) — compare blocking with the brand sets if you image very weak signals.
DX-BP405-10-25
405/10 nm laser-line bandpass filter
FF01-405/10-25 ET405/10x
  • Center wavelength (CWL): 405 nm
  • Bandwidth (FWHM): 10 nm
  • Peak transmission: 85%
  • Blocking: OD5
  • Size: Ø25.4 × 6 mm, mounted in ring
  • Typical measured (from curve): Peak T ≈ 90%; 50% points ≈ 401–410 nm
Semrock FF01-405/10-25 publishes FWHM 14.1 nm (10 nm guaranteed minimum bandwidth) and Tavg >87% (400–410 nm), OD>5, Ø25 × 3.5 mm. Ours: 10 nm FWHM, specified T 85% (typical measured peak ≈ 90%), OD5 — narrower passband, thicker 6 mm mount (check holder depth).
DX-BP488-10-25
488/10 nm laser-line bandpass filter
FF01-488/10-25 ZET488/10x
  • Center wavelength (CWL): 488 nm
  • Bandwidth (FWHM): 10 nm
  • Peak transmission: 90%
  • Blocking: OD5
  • Blocking range: 200–1100 nm
  • Size: Ø25.4 × 6 mm, mounted in ring
  • Typical measured (from curve): Peak T ≈ 94%; 50% points ≈ 485–493 nm
Semrock FF01-488/10-25 publishes Tavg >93% (483–493 nm), OD>4, FWHM 15.1 nm. Ours: specified T 90% (typical measured peak ≈ 94%), OD5 — higher stated blocking, narrower passband.
DX-BP400-40-25
400/40 nm bandpass filter
FF01-400/40-25 —
  • Center wavelength (CWL): 400 nm
  • Bandwidth (FWHM): 40 nm
  • Peak transmission: 90%
  • Blocking: OD5
  • Blocking range: 200–1100 nm
  • Size: Ø25.4 × 6 mm, mounted in ring
  • Typical measured (from curve): Peak T ≈ 98%; 50% points ≈ 376–419 nm
Semrock FF01-400/40-25 publishes Tavg >90% (380–420 nm) and OD>6. Ours: specified T 90% (typical measured plateau ≈ 97%), OD5 — Semrock specifies one OD more blocking.
DX-DI785R-25x36
785 nm Raman dichroic beamsplitter
LPD02-785RU-25x36 RT785rdc
  • Laser line: 785 nm
  • Reflection: R 99% @ 785 nm
  • Transmission band: 805–890 nm
  • Edge (50% transmission): ≈ 798 nm (typical measured)
  • Typical measured (from curve): T ≈ 97–99.7% from 805 to 890 nm; T ≈ 0.6% at 785 nm
  • Size: 25.2 × 35.6 × 1 mm
Chroma RT785rdc is 25.5 × 36 × 1 mm; ours is 25.2 × 35.6 × 1 mm — confirm fit in your holder. Measured edge ≈ 798 nm (50% T); AOI data on request.
DX-DI685LP-25x36
685 nm long-pass dichroic beamsplitter
FF685-Di02-25x36 T685lpxr
  • Edge wavelength: 685 nm (long-pass)
  • Reflection band: 610–660 nm (high reflection)
  • Transmission band: 700–800 nm (high transmission)
  • Typical measured (from curve): T ≈ 90–94% from 700 to 800 nm; T < 1% from 610 to 660 nm; 50% edge ≈ 685 nm
  • Size: 25.2 × 35.6 × 1 mm
Semrock FF685-Di02-25x36 publishes R>98% (350–676 nm) and T>93% (695–950 nm) — a wider specified range than ours (HR 610–660 nm, HT 700–800 nm). Chroma T685lpxr is 25.5 × 36 mm.

Curves: Typical measured transmission (manufacturer data, redrawn). Lot-specific test report supplied with each order. “Closest part” means the nearest published equivalent by wavelength and function; it does not imply identical performance. Brand specifications are quoted from their public product pages as published at the time of writing — check the brand's current datasheet for the latest values.

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Semrock and Chroma are trademarks of their respective owners; comparisons are based on published specifications. Dichroptics is not affiliated with, endorsed by, or sponsored by either company.

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